Sepax SRT-10 and SRT-10C SEC and Columns FPLC* and HPLC Compatible

Phase

SRT-10/10C SEC-100

SRT-10/10C SEC-300

SRT-10/10C SEC-500

SRT-10/10C SEC-1000

Material

Neutral, hydrophilic film bonded silica

Particle size

10 m

10 m

10 m

10 m

Pore size ()

~ 100

~ 300

~ 500

~ 1000

Protein MW range (native)

100 - 100,000

5,000 - 1,250,000

15,000 - 5,000,000

50,000 - 7,500,000

pH stability

2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)

Backpressure (7.8300 mm)

~ 300 psi (flow rate at 1.0 mL/min)

Maximum backpressure (psi)

~ 3,000

Salt concentration range

20 mM - 2.0 M

Maximum temperature (C)

~ 80

Mobile phase compatibility

Aqueous and organic

Column Volume

5 mL

14 mL

24 mL

105 mL

212 mL

490 mL

V-injection

5-100 L

15-250 L

25-940 mL

0.1-4.2 mL

0.2-8.5 mL

0.5-20 mL

Column Loading Guideline (BSA)

<= 1 mg

<= 2.5 mg

<= 9 mg

<= 42 mg

<= 85 mg

<= 235 mg

Standard Flow rate (Maximum)

0.35 mL/min

1.0 mL/min

1.65 mL/min (2.0 mL/min)

7.5 mL/min (10mL/min)

15 ml/min (25 ml/min)

41 ml/min (60 ml/min)

Typical Run Time

< 20 min

< 20 min

< 20 min

< 20 min

< 20 min

< 20 min

Back pressure

< 400 psi
< 30 bar
< 3 MPa

< 700 psi
< 50 bar
< 5 MPa

< 900 psi
< 60 bar
< 6 MPa

< 900 psi
< 60 bar
< 6 MPa

< 900 psi
< 60 bar
< 6 MPa

< 900 psi
< 60 bar
< 6 MPa