Sepax Nanofilm SEC Columns

Product Description

Nanofilm Technical Specifications

For biological separations, nonspecific interactions with the packing materials are the major contribution for low separation efficiency, such as peak tailing, as well as low recovery. The unique surface chemistry of Nanofilm SEC packings generates uniform polymer chains covalently bonded on the silica. Such a uniform stationary phase is neutral and hydrophilic, which has negligible nonspecific interactions with biological molecules, especially proteins. This special coating allows Nanofilm SEC columns to achieve high selectivity and high efficiency separations. For example, uracil, as a test compound could reach as high efficiency as 90,000 plates per meter, as shown in Table below.

Table 1. Column Efficiencies (Measured using uracil in 0.1 M phosphate buffer, pH 7.0)